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Reactions were performed with 50 µM RHKKAc, a fluorogenic peptide substrate from p53 residues 379–382, 500 µM NAD + co-substrate, and recombinant human SIRT-1 (NAD + -dependent) enzyme

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Science Composition & Standards Knowing what's actually in this stuff helps you understand why it's the go-to solvent for peptides

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